EFTA00317063.pdf

Individualized cancer care through molecular diagnostics Dr. Francis Barany Weill Cornell Medical College Confidential, October 2011 EFTA00317063 Our technology will guide physician decision in cancer prevention, early detection, and therapy based on the molecular signatures of the individual patient, rather than the average population. Confidential, October 2011 EFTA00317064  Cancer concerns • Are you or your family at risk of getting cancer? • Do you already have a hidden cancer growing within you? • What kind of treatment will you get? • Will your cancer return, and will you survive? Confidential, October 2011 EFTA00317065  Cancer concerns • Risk: Are you or your family at risk of getting cancer? • Early Detection: Do you already have a hidden cancer growing within you? • Individualized treatment: What kind of treatment will you get? • Recurrence and Metastasis: Will your cancer return, and will you survive? i odential, October 2011 EFTA00317066  Colorectal Cancer Progression Spread to other organs Early Stage Ill Detection Individualized 4 Recurrence & National Cancer Institute Confidential, October 2011 5 EFTA00317067  Major problems with current cancer prevention and treatment: • Individuals who will get cancer are not identified, losing the opportunity for prevention. • Those with early "hidden" cancers often find out too late for more effective treatment. • Individuals with cancer are given the average treatment for the average tumor with less than average results. • Chemotherapy rarely cures, but is very toxic. Confidential, October 2011 EFTA00317068  Addressing Cancer concerns • Risk: Identify individuals at risk of getting cancer by developing screens for changes in their DNA. • Early Detection: Identify molecular markers of cancer, and develop tools to detect them at an earlier and more treatable stage. • Individualized treatment: Identify tumor markers that predict outcome and guide treatment. • Recurrence and Metastasis: Develop cancer drugs to prevent recurrence and treat metastasis. Confidential, October 2011 EFTA00317069  The Distribution of Colorectal Cancer Sporadic Rare syndromes \ (70-80%) (<0.1%) / Familial adenomatous Polyposis (FAP) (H%) Hereditary nonpolyposis colorectal cancer (HNPCC) Familial (3-5%) (20-25%) Confidential, October 2011 EFTA00317070  Early Detection: Do you already have a hidden cancer within you? • Identify molecular markers of early cancers. • Develop tests for these changes to identify if you have hidden cancer. Confidential, October 2011 EFTA00317071 Challenges to Identify and Detect Early Cancer Create atlas of DNA Tumor DNA —> modifications & mutations 3O0000000c Find: 1 in 2 to Tissue biopsy 1 in 10. (colonoscopy) Tumor DNA )O300000o( Normal DNA DNA modifications .00000o0o( —> Find: 1 in 1,000 to )OX60000( X:60000OC‹ 1 in 10,000. Serum sample Tumor DNA )00000000( Do you Normal DNA DNA modifications & mutation have >C0000oax )CO=00( Find: 1 in 100 to colon Stool sample Bacterial DNA 1 in 1,000. cancer? )O00000UX 00.000000( )O00C00W O0000000( Confidential, October 2011 10 EFTA00317072  Limitations of current colon cancer early detection tests: • Colonoscopy: Compliance is less than 25%, cost is high, and 10%-20% of early lesions are missed. • Virtual colonoscopy: Requires similar uncomfortable prep work, high cost, and no benefit in accuracy. • Fecal occult blood test: Low cost, but misses too many colon cancers, and has mostly false results. • Epigenomics: "Single-marker" serum test has high false- positive rate: Of every 1,000 tests, it give 110 "positives", of which 1 will be a tumor, 109 will be erroneous and 1 tumor will be missed. Confidential, October 2011 EFTA00317073  Potential solution to colon cancer early detection test: • Identify multiple markers of colon cancer, where each marker covers 50% - 70% of tumors. • Develop novel microfabricated chip technology that would fully automate the assay, reducing cost and increasing accessibility of the test. • Develop new "multiplexed real-time" assay to identify up to a dozen markers directly from DNA purified from serum. • Low-cost test should achieve sensitivity and specificity that are significantly better than current approaches. Confidential, October 2011 12 EFTA00317074  The Fluidic Micro-processor for Analyzing Circulating DNA for Methylation Patterns Removal of Cellular Fraction from Whole Blood -* Serum i Affinity Selection of Methylated DNAs from Serum il Solid-Phase Pre-concentration of the Selected Circulating DNAs I Bisulfite Conversion of non-methylated C's into U's ii Purification of Bisultife Treated DNA I Run Multi-methyl-HRM Assay i Confidential, October 2011 13 EFTA00317075  Integrated Genotyping System using Modular Microfluidics Polycarbonate Module Cell Lysis Molecular Processing Flow Solid-Phase Extraction of Nucleic Acids Thermal Reactions (PCR, LDR) Fluidic Interconnect Microarray PMMA Module • Modular approach — match material to application • Continuous flow operation Confic October a 14 EFTA00317076  Strategic Advantage of iCareDx approach: • Multiplexed Assay format: Allows for greater than 95% specificity, overcoming the unacceptably high false test results inherent to single marker assays. • Multidimensional Assay: Allows for greater than 90% sensitivity, finding more individuals with hidden cancers than current one-dimensional assays. • Micro-fabricated devices: Allows for automated high- throughput processing of multiplexed assay, which is essential in a clinical setting. Confidential, October 2011 15 EFTA00317077  Principle Investigator: Francis Barany, Dr. Francis Barany received his Ph.D. in Microbiology in 1981 at The Rockefeller University with Professor Alexander Tomasz. He was a Helen Hay Whitney postdoctoral fellow with Professor Hamilton O. Smith at the Johns Hopkins University School of Medicine from 1982-1985. Upon appointment as an Assistant Professor in Microbiology at Weill Cornell Medical College in 1985, he was named a Cornell Scholar in Biomedical Sciences, and in 1992 received a five year Hirschl/ Monique Weill-Caulier Career Scientist Award. He currently holds the rank of Full Professor in the Department of Microbiology and Program of Biochemistry and Structural Biology at Cornell/Sloan Kettering Institute. He held adjunct appointments at The Rockefeller University in the Dept. of Chemistry, Biochemistry, and Structural Biology, as well as Director of Mutation Research at the Strang Cancer Prevention Center. A prolific inventor with 36 issued US patents, he is best known for developing the ligase chain reaction (LCR) and ligase detection reaction (LDR) and Universal DNA arrays for detection of infectious diseases, genetic diseases, and cancer-associated mutations. The Barany Laboratory patents and intellectual property have generated over $30 million in NIH Grants, NIST Grant, Industrial Sponsored Research Grants, over $10 million in royalties to Weill- Cornell, and close to $2 billion in sales to biotechnology companies. In 2009 he co-founded Coferon Inc., based on his invention of a new class of drugs that enter cells and self-assemble on the target. Dr. Barany was honored as Medical Diagnostics Research leader, Scientific American 50 in 2004, and the Ezra Innovation Award, Cornell University in 2011. Confidential, October 2011 16 EFTA00317078 Individualized cancer care through molecular diagnostics. Contact Information: Dr. Francis Barany Director Hidden Cancer Project Department of Microbiology & Immunology Weill Cornell Medical College 1300 York Avenue New York 10065 Office Mobile Fax —onfidential, October 2011 17 EFTA00317079 EFTA00317080